Liquid chromatography–mass spectrometry (LC-MS, or alternatively HPLC-MS) is an advanced chromatographic technology that combines the separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry (MS). LC-MS allows for multiple compound detection in a single injection it has both negative and positive polarity modes for increased detection. This is possible due to the high selectivity of compounds by identification of the mass to charge ratio that is compound specific allowing it to be used in a variety of applications. It is because of this technology of the LCMSMS that detection, identification and quantitation of precise compounds are discovered in complex matrices, natural and chemically treated materials without extensive sample preparation to remove interferences.
Liquid Chromatography is an analytical technique used for the separation of compounds that are soluble in organic or aqueous solutions. Trilogy Analytical Laboratory utilizes a variety of Liquid Chromatography (LC) equipment including High Performance Liquid Chromatography (HPLC), Ultra High Performance Liquid Chromatography (UHPLC), and Liquid Chromatography with Mass Selective Detection (LC/MS/MS). HPLC uses a high pressure pump to pass a liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. The mixture is separated into its components and detected using a variety of detectors including Fluorescent and Ultra-Violet detectors depending on the specific toxin. UHPLC equipment allows for a higher resolution, sensitivity, and faster analysis time over traditional HPLC.
The chromatography equipment utilized at Trilogy Analytical Lab consists of Gas Chromatography (GC) and several types of Liquid Chromatography (LC). Gas chromatography is a type of automated chromatography system (a technique used to separate mixtures of substances) in which the mixture to be analyzed is vaporized and carried by an inert gas through a special column and then to a detection device. The GC detection device used at Trilogy Lab is an Electron Capture Detection (ECD) used for the simultaneous analysis of a class of mycotoxins called Type A and B Trichothecenes (Deoxynivalenol, 3-Acetyl DON, 15-Acetyl DON, Nivalenol, Fusarenon X, T-2 Toxin, HT-2 Toxin, DAS, and Neosoleniol.